EFFECTS OF NICOTINE ON ENDOCYTOSIS AND INTRACELLULAR DEGRADATION OF HORSERADISH PEROXIDASE IN CULTIVATED MOUSE PERITONEAL MACROPHAGES
Identifieur interne : 003738 ( Main/Exploration ); précédent : 003737; suivant : 003739EFFECTS OF NICOTINE ON ENDOCYTOSIS AND INTRACELLULAR DEGRADATION OF HORSERADISH PEROXIDASE IN CULTIVATED MOUSE PERITONEAL MACROPHAGES
Auteurs : Johan Thyberg [Suède] ; Jan Nilsson [Suède]Source :
- Acta Pathologica Microbiologica Scandinavica Series A :Pathology [ 0108-0164 ] ; 1982-07.
English descriptors
- Teeft :
- Acid phosphatase activity, Acta path, Biochem, Control cells, Control medium, Cultured macrophages, Degradation, Dense core, Electron microscopy, Endocytosis, Golgi cisternae, Horseradish, Horseradish peroxidase, Human alveolar macrophages, Intracellular, Intracellular degradation, Intralysosomal, Karolinska institutet, Latex, Latex beads, Lysates, Lysosomal, Lysosomal enzymes, Lysosome, Macrophage, Medical risks, Methylamine, Nicotine, Osmium tetroxide, Peroxidase, Rinsed, Specific activities, Triplicate cultures, Vesicle.
Abstract
Thioglycollate‐elicited mouse peritoneal macrophages were cultivated in vitro in control medium or medium containing nicotine (1 nM‐1 μM). The drug caused a moderate lysosomal vacuolation and formation of vesicles with a dense core or ring. Furthermore, it partially inhibited uptake and intracellular degradation of horseradish peroxidase without affecting the specific activities of the lysosomal enzymes β‐glucuronidase and β‐N‐acetylglucosaminidase in lysates of the cells. A slight inhibitory effect on the phagocytosis of latex beads was also noted. Since nicotine was used in concentrations of the same magnitude that may be obtained in the blood after smoking one cigarette, it seems worthwhile to further explore these findings in relation to the medical risks of smoking.
Url:
DOI: 10.1111/j.1699-0463.1982.tb00098_90A.x
Affiliations:
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The drug caused a moderate lysosomal vacuolation and formation of vesicles with a dense core or ring. Furthermore, it partially inhibited uptake and intracellular degradation of horseradish peroxidase without affecting the specific activities of the lysosomal enzymes β‐glucuronidase and β‐N‐acetylglucosaminidase in lysates of the cells. A slight inhibitory effect on the phagocytosis of latex beads was also noted. Since nicotine was used in concentrations of the same magnitude that may be obtained in the blood after smoking one cigarette, it seems worthwhile to further explore these findings in relation to the medical risks of smoking.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement></list><tree><country name="Suède"><region name="Svealand"><name sortKey="Thyberg, Johan" sort="Thyberg, Johan" uniqKey="Thyberg J" first="Johan" last="Thyberg">Johan Thyberg</name></region><name sortKey="Nilsson, Jan" sort="Nilsson, Jan" uniqKey="Nilsson J" first="Jan" last="Nilsson">Jan Nilsson</name><name sortKey="Thyberg, Johan" sort="Thyberg, Johan" uniqKey="Thyberg J" first="Johan" last="Thyberg">Johan Thyberg</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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